Data Processing
MAC-tag GFP Controls:
Find Interactors
Data Filtering
Input file should be comma or tab separated SAINT output file
Parameters
Molecular Microscopy
Baits_for_ms-microscopyInput file should be comma separated SAINT output file and contain at least columns "Bait", "Prey", and "AvgSpec".
Remember to filter the data.
Example data for Molecular microscropy analysis:
Example_file_1ACGingras_proximity_biotinylation_map
About
This web application is developed and maintained by Varjosalo lab at the University of Helsinki . The MAC-tag method is described in our recent paper. Please refer to the paper for detailed description: https://www.nature.com/articles/s41467-018-03523-2
key references using MAC-tag approach and MS-microscopy:
- Liu, X. et al. Nature Communications. 208 p., 1188.
- Kondelin, J. et al. EMBO molecular medicine p., 8552
- Paakkola, T. et al. Human Molecular Gentics. 27p., 4288
- Yellapragada , V. et al. Frontiers in Endocrinology , vol. 10 , 48
- Keskitalo,S. et al. Frontiers in Immunology
Highlight in other review papers:
- Lossl, P. A . Nat Methods 15, 315, 2018
- Lundberg, E., Borner, G.H.H. Nat Rev Mol Cell Biol 20, 285-302,2019
- Laura Trinkle-Mulcahy.F1000 Research,2019
Contact information
Markku Varjosalo Ph.D, Docent
University of Helsinki
Institute of Biotechnology
Biocenter 3, 3rd floor
P.O.Box 65 (Viikinkaari 1)
FIN-00014 University of Helsinki Finland
